Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding.
PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin) is based on innovative high-capacity matrix for convenient single-step purifications of biotin and biotinylated substances. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin. The Resin consists of 90μm beads of cross-linked 6% agarose, to which Streptavidin has been coupled. Tests confirm that performance equals or exceeds popular Streptavidin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Per mL of streptavidin resin can capture 1 to 3 mg of biotinylated BSA or more than 120 nmol Biotin. PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin) contains PurKine™ Biotin-Tag Streptavidin Packed Column 6FF and buffers.There is no need for users to pack resin or prepare buffers themselves which can save precious time. In addition, because of the highly crosslinked resin, the tolerate linear flow rates of the kit is up to 500 cm/hour. It's totally ok for scaling up.