Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Affinity purified using solid phase DyLight 488, Goat Anti-Mouse IgG with finally > 95% purity based on SDS-PAGE. And Abbkine suggested starting 1:50-1:1,000 dilutions for most fluorescent applications.
DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 488, Goat Anti-Mouse IgG has higher cost performance than traditional FITC/Cy2 and Alexa 488.