Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. It is one of the PARP family of enzymes. PARP-1 involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks Mediates the poly(ADP-ribosyl)ation of APLF and CHFR .
Abbkine Cleaved-PARP-1 (D214) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the Internal region of human PARP-1. at AA rangle: 140-220. The antibody was tested to apply in ELISA and WB with Human and Mouse samples. The suggested starting dilutions are: WB: 1:500-1:2000, ELISA: 1:20000.
The Cleaved-PARP-1(D214) Polyclonal antibodie is performing very well for our research clinical trials. We used it on mouse osteosarcoma cells for clinical trials to verify results that we had gotten from an antibody from another manufacturer. The PARP-1 (D214) worked even better. It was able to detect PARP-1 . We first tried the recommended WB 1:500 concentration and it worked great. The signal produced was very strong and clear. The sensitivity of the PARP-1 (D214) was also perfect. Apart from the performance of the antibody, it was also great to be able to order the antibody in trial-sized quantities.