Apoptosis is a physiological process of cellular autodestruction. The central component of this machinery is a proteolytic system involving a family of proteases called caspases. Fas(APO-1/CD95) is a transmembrane receptor protein which induces apoptosis upon activation. In apoptosis triggered by Fas, a subset of caspases is activated. Among them, caspases-8 (FLICE/MACH) is the first step in the cas2 cade of apoptotic events induced by Fas. Cleavaged caspase-8 then activates other downstream caspases including caspase-9, thereby commiting the cell to undergo apoptosis. Many studies show that caspase-8 is related to the pathogenesis of certain diseases and injuries such as cancers, neurodegenerative disease, parasitosis, trauma and so on in human beings.
Abbkine Cleaved-Caspase-8 (D384) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the C-terminal region of human Caspase-8. at AA rangle: 310-390. The antibody is react with human and the verified applications are WB and ELISA. Abbkine suggested the starting dilutions are WB: 1:500-1:2000, ELISA: 1:40000.
Supplied as liquid , the concentration of the Cleaved-Caspase-8 (D384) antibody is 1mg/ml. Uniprot accession of the relative protein of the antibody is Q14790, and gene ID is 841. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.