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    Affinity of Protein A, Protein G and Protein A/G

    Thursday, 8 December 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Protein A, Protein G and Protein A/G are often used in antibody purification and other applications such as immunoprecipitation (IP) and chromatin immunoprecipitation(ChIP). Their binding abilities...

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    How to aliquot antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    The size of the aliquots will depend on how much one typically use in an experiment. Aliquots should be no smaller than 10 µl; the smaller the aliquot, the more the stock concentration is affected ...

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    How to store antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    We recommend storing antibody according to product manual.

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    How to choose suitable isotype control?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody ...

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    What’s the advantage of “IFKine” fluorescence secondary antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Compared to traditional fluorescence, “IFKine” fluorescence is brighter and more stable. In most immunofluorescence test, you need do double(multi)-labeled test. IFKine Green, IFKine Orange, IFKine...

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    How to choose suitable Secondary antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Which animal was used to generate your primary antibody? For instance, if your primary antibody is raised in a rabbit, you need to choose an anti-rabbit secondary antibody; if it is raised in a m...

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    About anti-Plant Actin Mouse Monoclonal Antibody (Cat.No. A01050)?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Reactivity: Arabidopsis, Nicotiana tabacum, Oryza sativa, Hordeum vulgare.Predicted to work with: Brassica napus, Gossypium herbaceum, Solanum tuberosum, Setaria italic, Triticum monococcum, Brachy...

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    What’s the concentration of Abbkine’s internal control antibodies and Tag antibodies?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    The concentration is usually 0.5-0.75mg/ml.

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    How to choose suitable internal control antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    Generally, the molecular weight of internal control protein and target protein should be more than 5kd. Whole cell protein: GAPDH, beta-actin, beta-tubulin. Nucleus protein: PCNA, Histone H3, Lamin...

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    How to choose suitable Primary antibody?

    Wednesday, 27 July 2016 By 夏洛特 at Frances Hunt Furniture News Blog in General FAQs

    The species of sample and experiment application are very important when choosing Primary antibody. Please choose Primary antibody according to the “Reactivity” and “Applications”.

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