Protein A, Protein G and Protein A/G are often used in antibody purification and other applications such as immunoprecipitation (IP) and chromatin immunoprecipitation(ChIP). Their binding abilities...
The size of the aliquots will depend on how much one typically use in an experiment. Aliquots should be no smaller than 10 µl; the smaller the aliquot, the more the stock concentration is affected ...
We recommend storing antibody according to product manual.
Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody ...
Compared to traditional fluorescence, “IFKine” fluorescence is brighter and more stable. In most immunofluorescence test, you need do double(multi)-labeled test. IFKine Green, IFKine Orange, IFKine...
Which animal was used to generate your primary antibody? For instance, if your primary antibody is raised in a rabbit, you need to choose an anti-rabbit secondary antibody; if it is raised in a m...
Reactivity: Arabidopsis, Nicotiana tabacum, Oryza sativa, Hordeum vulgare.Predicted to work with: Brassica napus, Gossypium herbaceum, Solanum tuberosum, Setaria italic, Triticum monococcum, Brachy...
What’s the concentration of Abbkine’s internal control antibodies and Tag antibodies?
in General FAQs
The concentration is usually 0.5-0.75mg/ml.
Generally, the molecular weight of internal control protein and target protein should be more than 5kd. Whole cell protein: GAPDH, beta-actin, beta-tubulin. Nucleus protein: PCNA, Histone H3, Lamin...
The species of sample and experiment application are very important when choosing Primary antibody. Please choose Primary antibody according to the “Reactivity” and “Applications”.