What is immunofluorescence?
Immunofluorescence (IF) technology is based on the principle of antigen-antibody reaction, first labeling known antigens or antibodies with fluorescent groups, and then using this fluorescent antibody or antigen as a probe to detect the corresponding antigens or antibodies in cells or tissues. It is a technology established on the basis of immunology, biochemistry and microscope technology. The principle of immunofluorescence The basic reaction of immunology is the antigen-antibody reaction. Because the antigen-antibody reaction has a high degree of specificity, when an antigen-antibody reaction occurs, as long as one of the factors is known, the other factor can be detected. Immunofluorescence technology is to label antibodies (or antigens) with fluorescent pigments that do not affect the activity of antigens and antibodies,…
Using Abbkine CCK-8 to analyze cell toxicity
Cell toxicity is caused by exogenous toxicant which can damage cells, especially when the toxicant can cause cell death and serious organ dysfunction. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. The short name of Cell Counting Kit-8 is CCK-8 Kit, which is established based on WST-8 reaction and widely used for cell proliferation/Toxicity, drug screening as well as tumor drug sensitivity test experiment, etc... Abbkine CCK-8 Kit provides one ready-to-use CCK-8 solution, it is convenient and fast which will be your prefect choice to replace MTT method. Figure.1 The reaction mechanism of CCK-8(WST-8) Picture Source: https://www.creative-bioarray.com/support/colorimetric-cell-viability-assay.htm Compared with traditional method for Cell Toxicity/Proliferation experiment, CCK-8 has many…
New arrival：ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues
The new products of Abbkine is coming! We have launched 3 new products, including 1 protein extraction kit and 2 biochemical detection kits. The details are as follows： Catalog no Product name Size and Unit KTP3007 ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues 5T/50T KTB1123 CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit 48T/96T KTB1124 CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit 48T/96T The column method protein extraction kit is mainly introduced! When you were extracting protein from cells and tissues, do you have any troubles with proteins loss? Are you tired of the tedious and persistent experimental operation steps? This new product from Abbkine is for you! The magic of it is that it can not only…
How to analyze the fluorescence intensity of cells by using Image J?
Today we're going to talk about Image J’s use in immunohistochemistry. Image J software is completely free and easy to download. As a scientific researcher, we face all kinds of maddening experimental data every day. If we can skillfully use Image J, it will undoubtedly be very helpful for us to analyze the experimental results. Now let's introduce how to analyze immunohistochemistry by Image J. Here is an example: Step 1: Import Image: Open Image J, File→Open: Open the Image to be measured Step 2: Transform the Image format: Image→Type→RGB Stack At this time, a scroll bar appears at the bottom of the picture. Files 1, 2, and 3 correspond to red, green, and blue respectively. Select relatively clear pictures…
Alkaline phosphatase and Bone Metabolism
AKP / ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP / ALP is widely distributed in various organs of the human body, mainly the liver. An alkaline phosphatase level test (ALP test) measures the amount of alkaline phosphatase enzyme in your bloodstream. The test requires a simple blood draw and is often a routine part of other blood tests. An ALP test may be performed to determine how well your liver and gallbladder are functioning or to identify problems with your bones. Figure 1: Smart nanoprobes in the detection of ALP(Picture Source: https://pubs.rsc.org ) Bone and liver alkaline phosphatases are the predominant alkaline phosphatase (ALP) isoenzymes in serum…
Abbkine mitochondrial respiratory chain complex series detection kits and tricarboxylic acid cycle series detection kits
Some time ago, Abbkine launched a lot of rare treasures with full horsepower, and quickly captured the hearts of a large number of heroes and knights on the rivers and lakes, and received rave reviews. To this end, the editor deliberately shows the members who have recently debuted in the center of the traffic. Type Item No. Product Name Flow Explosion Series BMU101-EN SuperKine™ West Pico PLUS Chemiluminescent Substrate BMU102-EN SuperKine™ West Femto Maximum Sensitivity Substrate BMU103-EN SuperKine™ Enhanced Antibody Dilution Buffer KTD101-EN Universal Loading Control Antibody Cocktail Mitochondrial respiratory chain complex series KTB1850 CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) KTB1860 CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) KTB1880…
Promotion of the cell status detection kits
Do you have any trouble in the process of exploring the cell's proliferation, aging, and apoptosis? Perhaps there are some concerns about that weak fluorescence will interfere with your judgment? Or did the cumbersome steps block your thoughts? Annexin V of AbFluor™ - Increases Fluorescence Intenacity by 30% or More! It saves half of the operation time with the EDU Kit! During this summer, the Abbkine's cell state assay kits will "witness" the vitality, apoptosis and aging in the world of cells with you! Promotion participants: Authorized Distributors Overseas. Promotion time: From now to 30th September, 2021. Promotion products: Cell status detection in the attached list (Table 1). Promotion Discount: 55% off. Table 1: Product Name Cat. No…
New arrival |【sRANKL】Recombinant human soluble RANK ligand
【sRANKL】Recombinant human soluble RANK ligand Introduction to sRANKL Tumor necrosis factor ligand superfamily member 11, also known as nuclear factor receptor activator kappa-B ligand, osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, expressed in a variety of cells, including osteogenic Cells, fibroblasts, activated T cells and bone marrow stromal cells can interact with the decoy receptor OPG. TNFSF11 participates in many basic biological processes, such as regulating the interaction between T cells and dendritic cells, regulating T cell-dependent immune responses and enhancing bone resorption in humoral hypercalcemia of malignant tumors. TNFSF11 also enhanced the ability of dendritic cells to stimulate the proliferation of naive T cells. Product information Related product information Product Name Item No. Mouse RANKL protein PRP1114 …
New arrival |【FGF21】Recombinant Human Fibroblast Growth Factor 21
【FGF21】Recombinant Human Fibroblast Growth Factor 21 Introduction to FGF21 FGF21 is a member of the Fibroblast Growth Factor (FGFs) family. The proteins of this family have a wide range of mitogenic and cell survival activities, and are involved in a variety of biological processes, such as angiogenesis, wound healing, neurodevelopment, and tumor occurrence and development. Most FGFs (FGF3-8, 10, 15, 17-19, 21-23) have typical signal sequence secreted proteins at the N-terminus. Among them, Fibroblast Growth Factor 21 (FGF 21) is specific in the liver Sexual expression, reducing blood sugar and triacylglycerols through the scope of adipose tissue and pancreas. Glucagon improves the function of pancreatic islet B cells, thereby preventing diet-induced obesity and insulin resistance. In addition, FGF21 has also…
New arrival |【Annexin V/ANXA5】Recombinant Human Annexin A5
【Annexin V/ANXA5】Recombinant Human Annexin A5 Introduction to Annexin V Annexin V is a calcium ion-dependent phospholipid binding protein with a molecular weight of 35~36KDa, which can specifically bind to phosphatidylserine PS with high affinity. In normal cells, phosphatidylserine is only distributed on the inner side of the lipid bilayer of the cell membrane. In the early stage of cell apoptosis, the membrane phosphatidylserine (PS) turns from the inner side of the lipid membrane to the outer side. As a phospholipid binding protein, Annexin V has a high affinity with phosphatidylserine. It binds to the cell membrane of cells in the early stage of apoptosis through the phosphatidylserine exposed on the outside of the cell. Therefore, Annexin V is a sensitive…
