AR (androgen receptor) is a member of the steroid superfamily of ligand-dependent transcription factors. They regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. AR consists of an amino terminal modulating domain, a central DNA-binding domain, a hinge region and a carboxyl terminal ligand binding domain. Defects in AR are the cause of androgen insensibility syndrome (genital disorders), X-linked spinal and bulbar muscular atrophy (Kennedy disease), metastatic prostate cancer and infertility male syndrome.
AR Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000. Not yet tested in other applications.
The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. AR Polyclonal Antibody worked very well. I am satisfied with the results of the experiment.