RFP (Red Fluorescent Protein), cloned from Discosoma coral (DsRed, DsRed2 or drFP583), is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
Anti-RFP Tag Mouse Monoclonal Antibody (9D1) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been routinely tested with WB application. And Abbkine suggested starting dilution is as follows: WB 1:5000.
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