A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, where N stands for Amino-terminus and C stands for Carboxy terminus. It can be fused to the C-terminus and the N-terminus of a protein. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.
Anti-Myc Tag Mouse Monoclonal Antibody (2D5) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB, IP and IF. And Abbkine suggested starting dilutions are as follows: WB 1:5000, IP: 1:200, IF: 1:1000.
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