mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.
Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been routinely tested with WB application. And Abbkine suggested starting dilution is as follows: WB 1:5000.
The mCherry Tag Antibody can identify recombinant protein marked by mCherry that have alread been expressed, including those in the amino acid and the carboxyl terminal. Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) worked very well. I am satisfied with the results of the experiment.