Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus "housekeeping genes" are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight with the protein of interest so that the bands are distinct and expression levels quantifiable. The most popular loading control detection antibody for Nucleoprotein is Anti-Histone H3 antibody. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes and the histone gene is highly conserved.
Abbkine's Anti-Histone H3 Monoclonal Antibody(2D9) was affinity-purified from mouse ascites by affinity-chromatography using specific Synthetic Peptide. This product is specifically apply in WB, detecting Zebrafish samples. Abbkine suggest the starting dilution is 1:1000-3000.
Usually it's very difficult to find out antibodies that could detect Zebrafish samples in market. I'm very lucky to find Abbkine's Anti-Histone H3 Monoclonal Antibody(2D9) and it worked very well as a loading control antibody. By the way, I love its price.