A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Anti-His Tag Mouse Monoclonal Antibody (5C3) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with IF, IP and WB. Abbkine suggested starting dilutions are as follows: WB 1:3000, IP: 1:200, IF: 1:1000.
His tag is a short peptide composed of 6 histidine (His-His-His-His-His-His). Because its molecular weight is small, His tag is relatively easy to isolate and purify. I chose Anti-His Tag Mouse Monoclonal Antibody (5C3) to detect the expression of His tag fusion protein. I am satisfied with the result.