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Anti-His Tag Mouse Monoclonal Antibody (5C3) Review

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other a02050-wbprokaryotic expression systems.

Anti-His Tag Mouse Monoclonal Antibody (5C3)  was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with IF, IP and WB. Abbkine suggested starting dilutions are as follows: WB 1:3000, IP: 1:200, IF: 1:1000.

His tag is a short peptide composed of 6 histidine (His-His-His-His-His-His). Because its molecular weight is small, His tag is relatively easy to isolate and purify. I chose Anti-His Tag Mouse Monoclonal Antibody (5C3) to detect the expression of His tag fusion protein. I am satisfied with the result.

Anti-His Tag Mouse Monoclonal Antibody (5C3)
  • Editor Rating

  • Rated 5 stars
  • Spectacular
$90

  • Product Features
    Editor: 100%
  • Usage Performance
    Editor: 100%
  • Price Advantage
    Editor: 95%

Review Summary:

His tag is a short peptide composed of 6 histidine (His-His-His-His-His-His). Because its molecular weight is small, His tag is relatively easy to isolate and purify. I chose Anti-His Tag Mouse Monoclonal Antibody (5C3) to detect the expression of His tag fusion protein. I am satisfied with the result.