Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology.
Anti-GFP Tag Mouse Monoclonal Antibody (3D3) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB applications. And Abbkine suggested starting dilutions are as follows: WB 1:5000.
Green fluorescent protein is not only non-toxic, but also self illuminated without other coenzyme. We can study the dynamic process of living cells at the molecular level. Anti-GFP Tag Mouse Monoclonal Antibody (3D3) works well. This product is what I want.