FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Anti-Flag Tag Mouse Monoclonal Antibody (1B10) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB, IP and IF applications. And Abbkine suggested starting dilutions are as follows: WB 1:5000, IP: 1:200, IF: 1:2000.
Flag Tag is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Anti-Flag Tag Mouse Monoclonal Antibody (1B10) is one of the most representative products. It tested with WB, IP and IF applications. Particularly, the price is lovely!