Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy. Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP).
Anti-EYFP-Tag Monoclonal Antibody(10T3) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB. And Abbkine suggested starting dilutions are as follows: WB 1:5000.
Like green fluorescent protein (GFP), YFP is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. I am happy to choose Anti-EYFP-Tag Monoclonal Antibody(10T3). The pictures are beautiful!