CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins.
Anti-CBP Tag Monoclonal Antibody(12H5) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB. And Abbkine suggested starting dilutions are as follows: WB 1:5000.
The CBP tag binds to calmodulin resin in the presence of low concentration of calcium and can be eluted with a neutral buffer. The antibody is suitable for detecting CBP fusion proteins. The quality of the Anti-CBP Tag Monoclonal Antibody(12H5) is found satisfactory. I am very happy that I chose this product.