AmCyan is one of the existing violet fluorochromes for use in flow cytometers equipped with a violet (405 nm) laser. It is also widely used as a label in fluorescent spectroscopy. AmCyan was originally derived from the fluorescent protein amFP486. AmFP486 was cloned from the sea anemone Anemonia majano (GenBank accession number AF168421), and belongs to the family of fluorescent proteins (FPs) isolated from coral reef organisms; green fluorescent protein (GFP), derived from the jellyfish Aequorea victoria, is its most famous representative.AmCyan has been
adapted from the corresponding full length cDNA for higher solubility, brighter emission, and rapid chromophore maturation. AmCyan1 has been human-codon optimized for enhanced translation in mammalian cells.
Anti-AmCyan Monoclonal Antibody (8T2) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB. And Abbkine suggested starting dilutions are as follows: WB 1:5000.
AmCyan is a very good blue fluorescent reporter protein, and useful in two-color analyses with DsRed2, DsRed-Express, or AsRed2, and in three-color analyses with ZsYellow and HcRed. I've tried Anti-AmCyan Monoclonal Antibody (8T2) with excellent results. I am a loyal fan of Abbkine！