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Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)

Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)

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Specification

Product name Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)
Immunogen Synthetic Peptide
Host Mouse
Applications IF, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative Vesicular stomatitis virus glycoprotein tag; VSV tag; VSV-G epitope; VSVG; vsvg tag

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.
Alternative Vesicular stomatitis virus glycoprotein tag; VSV tag; VSV-G epitope; VSVG; vsvg tag

Image & description

Fig.1. Immunofluorescence staining (1:1000) of VSV-G fusion protein in 293 cells with red and counterstained with DAPI.

Fig.1. Immunofluorescence staining (1:1000) of VSV-G fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. IP (1:200)-WB (1:5000) analysis of VSV-G Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with VSV-G tag protein (lane B); IP untransfected 293 cell lysate with Anti VSV-G tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti VSV-G tag mAb (lane E).

Fig.2. IP (1:200)-WB (1:5000) analysis of VSV-G Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with VSV-G tag protein (lane B); IP untransfected 293 cell lysate with Anti VSV-G tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti VSV-G tag mAb (lane E).

Fig.1. Western blot analysis of 1ug VSV-G fusion protein with Anti-VSV-G mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions.

Fig.1. Western blot analysis of 1ug VSV-G fusion protein with Anti-VSV-G mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions.

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