Sheep Haptoglobin (HP) ELISA Kit

Product Information

  • Product name

    Sheep Haptoglobin (HP) ELISA Kit

  • Reactivity

    Sheep

  • Applications

    ELISA

  • Application notes

    This Sheep Haptoglobin (HP) ELISA Kit employs a two-site sandwich ELISA to quantitate HP in samples. An antibody specific for HP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HP bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Serum, Plasma, Other biological fluids

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Product Properties

  • Features & Benefits

    Sheep Haptoglobin (HP) ELISA Kit has high sensitivity and excellent specificity for detection of Sheep HP. No significant cross-reactivity or interference between Sheep HP and analogues was observed.

  • Limit of detection

    Request Information

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Haptoglobin is a protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). In clinical settings, the haptoglobin assay is used to screen for and monitor intravascular hemolytic anemia. In intravascular hemolysis free hemoglobin will be released into circulation and hence haptoglobulin will bind the Hb. This causes a decline in Hp levels. Conversely, in extravascular hemolysis the reticuloendothelial system, especially splenic monocytes, phagocytose the erythrocytes and hemoglobin is not released into circulation and hence haptoglobulin levels are normal.

  • Gene ID

    3240

  • Alternative names

    HP; BP; HP2-ALPHA-2; HPA1S; MGC111141; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin; alpha polypeptide; haptoglobin; beta polypeptide

  • Accession

    P00738

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTE110063

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