PurKine™ Protein L Packed Column 4FF

Product Information

  • Product name

    PurKine™ Protein L Packed Column 4FF

  • Application notes

    PurKine™ Protein L Resin 4FF has been designed to purify of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein L has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein L resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Product Properties

  • Formulation

    Liquid solution with 50% slurry in 20% ethanol.

  • Features & Benefits

    • High capacity—More than 15mg human lgG per mL of resin.
    • High performance—Recombinant Protein L is produced in E.coli.It binds Igs through interactions with the light kappa chains.Protein L binds a wider range of Ig classes than Protein A or G. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.
    • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
    • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
    • Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.

  • Storage buffer

    Liquid in PBS buffer containing 20% ethanol.

  • Storage instructions

    Stable for one year at 2-8°C from date of shipment. Do not freeze.

  • Shipping

    Blue ice

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    PurKine™ Protein L Purification system is effective for affinity purification of mammalian IgG that contain specific kappa light chains from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein L is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are present in the media serum supplement. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein L Purification Resin is also available as prepacked spin column and kit formats.

  • Alternative names

    Protein L; Protein L resin

Reviews

There are no reviews yet.

Be the first to review “PurKine™ Protein L Packed Column 4FF”

Your email address will not be published. Required fields are marked *

PurKine™ Protein L Packed Column 4FF
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: BMC20804

  • Size
  • Price
  • 1ml×5 $316
  • 3ml×5 $758

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

Gaining Your Trust with our

High quality *
Attractive price
100% Guarantee
Secured shipment