Mouse VEGF164 protein

Product Information

  • Product name

    Mouse VEGF164 protein

  • Sequence

    Amino acid sequence derived from mouse VEGF164 (isoform VEGF-1) (Q00731-2) (Met 1-Arg 190) was expressed and purified.

  • Activity

    Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.8-4 ng/mL.

  • Protein length

    The recombinant mouse VEGF164 consists of 164 amino acids and has a calculated molecular mass of 19.4 kDa. It migrates as an 24 kDa band in SDS-PAGE under reducing conditions.

  • Preparation method

    Baculovirus-Insect Cells

  • Purity

    > 90 % as determined by SDS-PAGE

Fig.SDS-PAGE analysis of Mouse VEGF164 protein.

Product Properties

  • Formulation

    Lyophilized from sterile 20mM HAC-NaAC, 500mM NaCl, pH 6.5

  • Molecular weight

    19.4 kDa

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse VEGF164 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized Mouse VEGF164 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

  • Gene ID

    22339

  • Alternative names

    Vegf; Vpf; Vascular endothelial growth factor A; VEGFA

  • Others

    The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method

  • Accession

    Q00731

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP100386

  • Size
  • Price
  • 5µg $120
  • 20µg $410
  • 100µg $1240
  • 1mg $6240

Contact information

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