Mouse Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) ELISA Kit

Product Information

  • Product name

    Mouse Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) ELISA Kit

  • Reactivity

    Mouse

  • Applications

    ELISA

  • Application notes

    This Mouse Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF11A in samples. An antibody specific for TNFRSF11A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF11A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF11A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF11A bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Mouse Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Product Properties

  • Kit components

    • Mouse Tumor necrosis factor receptor superfamily member 11A microplate
    • Mouse Tumor necrosis factor receptor superfamily member 11A standard
    • Mouse Tumor necrosis factor receptor superfamily member 11A detect antibody
    • Streptavidin-HRP
    • Standard diluent
    • Assay buffer
    • HRP substrate
    • Stop solution
    • Wash buffer
    • Plate covers

  • Features & Benefits

    Mouse Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF11A. No significant cross-reactivity or interference between Mouse TNFRSF11A and analogues was observed.

  • Calibration range

    37.5-2400 pg/mL

  • Limit of detection

    39.16 pg/mL

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    RANk is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin ligand (OPGL)-induced osteoclastogenesis in osteoclast precursor cells. Northern blot analysis indicated that the 4.5-kb human RANK mRNA is expressed ubiquitously. In some human cell lines, the authors detected additional transcripts that were derived from the use of alternative polyadenylation signals in the RANK gene.

  • Gene ID

    21934

  • Alternative names

    TNFRSF11A; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; loss of heterozygosity; 18; chromosomal region 1; osteoclast differentiation factor receptor; receptor activator of nuclear factor-ka

  • Accession

    O35305

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTE70130

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