LinKine™ HRP Labeling Kit

Product Information

  • Product name

    LinKine™ HRP Labeling Kit

  • Application notes

    LinKine™ HRP Labeling Kit provides a simple and quick process to conjugate antibodies, peptides, proteinsand other molecules with free amine groups with HRP.

  • Conjugate

    HRP

Fig. LinKine™ HRP Labelling Kit is designed for preparing HRP conjugates directly from proteins, peptides, and other ligands that contain a free amino group.

Product Properties

  • Kit components

    • Activated HRP conjugates solution • HRP labeling solution • HRP quencher powder

  • Features & Benefits

    • Activated HRP – periodate-treated, aldehyde-activated horseradish peroxidase, ready for Labeling to antibodies and other proteins at sites of primary amines (e.g., lysines). • High activity HRP – enzyme activity is 120 to 200 units/mg; activated enzyme is stable for at least 12 months at -20°C. • Convenient quantities – each 1 mg-quantity of activated enzyme is sufficient for reaction with 1 mg of IgG. • Customizable – vary the molar ratios, reaction buffer and pH, and other parameters to acheive conjugates with different levels of HRP incorporation and activity.

  • Usage notes

    • The purified antibody to be labeled should ideally be in 10-50mM amine-free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5.
    • Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g. EDTA), and sugars have no effect on Labeling efficiency. We do not recommend performing a HRP Labeling in presence of sodium azide or adding sodium azide with your HRP conjugated antibody. Glycerol up to 50% has no effect.
    • The amount of antibody used for Labeling should ideally correspond to molar ratios between 1:4 and 1:1 Antibody to HRP.
    • Antibody concentrations in the range 0.5-4 mg/ml generally give optimal results.

  • Storage instructions

    Store kit components at 4˚C for 6 months.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch.

  • Alternative names

    Horseradish peroxidase; HRP

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LinKine™ HRP Labeling Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTL0100

  • Size
  • Price
  • 3*20 μg $100
  • 100 μg $120
  • 3*100 μg $140
  • 1 mg $220

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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