| Product name | Human S100 calcium binding protein A14 (S100A14) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human S100 calcium binding protein A14 (S100A14) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A14 in samples. An antibody specific for S100A14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A14 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | S100A14; BCMP84; S100A15; OTTHUMP00000032965; OTTHUMP00000032966; breast cancer membrane protein 84 |
| Kit components | • Human S100 calcium binding protein A14 microplate • Human S100 calcium binding protein A14 standard • Human S100 calcium binding protein A14 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human S100 calcium binding protein A14 (S100A14) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A14. No significant cross-reactivity or interference between Human S100A14 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | S100A14 is a member of a subfamily of proteins related by Ca(2+)-binding motifs to the EF-hand Ca(2+)-binding protein superfamily.Using a proteomics approach to identify genes upregulated in breast cancer, followed by database analysis and PCR of a breast carcinoma cell line, Adam et al. (2003) cloned S100A14, which they called BCMP84. BCMP84 expression in normal tissue was restricted to stratified squamous epithelium of skin, cervix, and tonsil. Weak staining was seen in normal breast ductal tissue, but strong immunoreactivity was detected in 10 of 58 (17%) breast cancer tissues. BCMP84 was expressed in the plasma membrane of transiently transfected breast carcinoma cells.By fluorescence in situ hybridization, Pietas et al. (2002) mapped the S100A14 gene to chromosome 1q21 in a cluster with at least 15 other S100 genes. |
| Gene ID | 57402 |
| Alternative | S100A14; BCMP84; S100A15; OTTHUMP00000032965; OTTHUMP00000032966; breast cancer membrane protein 84 |
| Accession | Q9HCY8 |
Fig.1. Human S100 calcium binding protein A14 (S100A14) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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