| Product name | Human Putative helicase MOV-10 (MOV10) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Putative helicase MOV-10 (MOV10) ELISA Kit employs a two-site sandwich ELISA to quantitate MOV10 in samples. An antibody specific for MOV10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOV10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOV10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOV10 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MOV10; RP11-426L16.2; DKFZp667O1423; FLJ32791; KIAA1631; fSAP113; gb110; Mov10; Moloney leukemia virus 10; homolog; OTTHUMP00000013679; OTTHUMP00000013680; functional spliceosome-associated protein 113 |
| Kit components | • Human Putative helicase MOV-10 microplate • Human Putative helicase MOV-10 standard • Human Putative helicase MOV-10 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Putative helicase MOV-10 (MOV10) ELISA Kit has high sensitivity and excellent specificity for detection of Human MOV10. No significant cross-reactivity or interference between Human MOV10 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MOV10 (Mov10 RISC Complex RNA Helicase) is a Protein Coding gene. Among its related pathways are RET signaling and Immune System. GO annotations related to this gene include poly(A) RNA binding and helicase activity. An important paralog of this gene is MOV10L1. Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription. |
| Gene ID | 4343 |
| Alternative | MOV10; RP11-426L16.2; DKFZp667O1423; FLJ32791; KIAA1631; fSAP113; gb110; Mov10; Moloney leukemia virus 10; homolog; OTTHUMP00000013679; OTTHUMP00000013680; functional spliceosome-associated protein 113 |
| Accession | Q9HCE1 |
Fig.1. Human Putative helicase MOV-10 (MOV10) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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