Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit

Product Information

  • Product name

    Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit

  • Reactivity

    Human

  • Applications

    ELISA

  • Application notes

    This Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA5 in samples. An antibody specific for PSMA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human Proteasome subunit alpha type-5 (PSMA5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Product Properties

  • Kit components

    • Human Proteasome subunit alpha type-5 microplate
    • Human Proteasome subunit alpha type-5 standard
    • Human Proteasome subunit alpha type-5 detect antibody
    • Streptavidin-HRP
    • Standard diluent
    • Assay buffer
    • HRP substrate
    • Stop solution
    • Wash buffer
    • Plate covers

  • Features & Benefits

    Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMA5. No significant cross-reactivity or interference between Human PSMA5 and analogues was observed.

  • Calibration range

    0.312-20 ng/mL

  • Limit of detection

    0.124 ng/mL

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.The cDNA encoded a 241-amino acid polypeptide. The calculated and observed molecular masses were 26 kD and 29 kD, respectively. Northern blot analysis revealed an mRNA of 1.2 kb in both human placenta and HeLa cells.The alpha subunits comprise the outer rings of the proteasome. Some alpha subunits contain a functional nuclear localization signal; proteasomes are found in both the nuclear and cytoplasmic compartments of the cell. Alpha subunits may constitute a physical barrier that limits access of cytosolic proteins into the inner proteolytic chamber.

  • Gene ID

    5686

  • Alternative names

    PSMA5; MGC117302; MGC125802; MGC125803; MGC125804; PSC5; ZETA; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome alpha 5 subunit; proteasome component 5;

  • Accession

    P28066

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Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTE61085

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