| Product name | Human Prickle-like protein 2 (PRICKLE2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Prickle-like protein 2 (PRICKLE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE2 in samples. An antibody specific for PRICKLE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PRICKLE2; DKFZp686D143; DKFZp686H1748; DKFZp686M031; prickle-like 2 |
| Kit components | • Human Prickle-like protein 2 microplate • Human Prickle-like protein 2 standard • Human Prickle-like protein 2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Prickle-like protein 2 (PRICKLE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRICKLE2. No significant cross-reactivity or interference between Human PRICKLE2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | By searching an EST database for sequences similar to those of Drosophila and Xenopus Prickle, Katoh and Katoh (2003) identified human PRICKLE2. The deduced 844-amino acid protein contains an N-terminal PET domain followed by 3 LIM domains and a C-terminal prickle homology domain. PRICKLE2 shares 51.9% overall identity with PRICKLE1 and 79.3% identity within the N-terminal PET and LIM domains. EST database analysis revealed that PRICKLE1 and PRICKLE2 are coexpressed in brain, eye, and testis; additionally, PRICKLE2 is expressed in fetal brain, adult cartilage, pancreatic islet, gastric cancer, and uterine tumor.Katoh and Katoh (2003) determined that the PRICKLE2 gene contains at least 8 exons and that the 5-prime untranslated region is interrupted by intron 1. |
| Gene ID | 166336 |
| Alternative | PRICKLE2; DKFZp686D143; DKFZp686H1748; DKFZp686M031; prickle-like 2 |
| Accession | Q7Z3G6 |
Fig.1. Human Prickle-like protein 2 (PRICKLE2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.