| Product name | Human Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA Kit employs a two-site sandwich ELISA to quantitate NBL1 in samples. An antibody specific for NBL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNBL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | NBL1; RP5-1056L3.4; D1S1733E; DAN; DAND1; MGC8972; NB; NO3; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region; suppression of tumorigenicity 1; neuroblastoma sup |
| Kit components | • Human Neuroblastoma suppressor of tumorigenicity 1 microplate • Human Neuroblastoma suppressor of tumorigenicity 1 standard • Human Neuroblastoma suppressor of tumorigenicity 1 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NBL1. No significant cross-reactivity or interference between Human NBL1 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | NBL1 product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for NBL1. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist) is a Protein Coding gene. Diseases associated with NBL1 include Neuroblastoma and Regular Astigmatism. Among its related pathways are TGF-beta signaling pathway. GO annotations related to this gene include protein homodimerization activity and morphogen activity. An important paralog of this gene is MINOS1-NBL1. |
| Gene ID | 100532736 |
| Alternative | NBL1; RP5-1056L3.4; D1S1733E; DAN; DAND1; MGC8972; NB; NO3; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region; suppression of tumorigenicity 1; neuroblastoma sup |
| Accession | P41271 |
Fig.1. Human Neuroblastoma suppressor of tumorigenicity 1 (NBL1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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