Human Brucella Antibody IgM (Brucella-Ab-IgM) ELISA Kit

Product Information

  • Product name

    Human Brucella Antibody IgM (Brucella-Ab-IgM) ELISA Kit

  • Reactivity


  • Applications


  • Application notes

    This Human Brucella Antibody IgM (Brucella-Ab-IgM) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brucella-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Brucella-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled Brucella-Ab-IgM and unlabeled Brucella-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Brucella-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.

  • Detection method


  • Sample type

    Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human Brucella Antibody IgM (Brucella-Ab-IgM) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Product Properties

  • Kit components

    • Human Brucella Antibody IgM microplate
    • Human Brucella Antibody IgM standard
    • Human Brucella Antibody IgM detect antibody
    • Streptavidin-HRP
    • Standard diluent
    • Assay buffer
    • HRP substrate
    • Stop solution
    • Wash buffer
    • Plate covers

  • Features & Benefits

    Human Brucella Antibody IgM (Brucella-Ab-IgM) ELISA Kit has high sensitivity and excellent specificity for detection of Human Brucella-Ab-IgM. No significant cross-reactivity or interference between Human Brucella-Ab-IgM and analogues was observed.

  • Calibration range

    37.5-2400 pg/mL

  • Limit of detection

    31.76 pg/mL

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 µm), nonencapsulated, nonmotile, facultatively intracellular coccobacilli. Brucella is the cause of brucellosis, which is a zoonosis transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. Transmission from human to human, for example through sexual intercourse or from mother to child, is exceedingly rare, but possible. Minimum infectious exposure is between 10 and 100 organisms.

  • Alternative names


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Human Brucella Antibody IgM (Brucella-Ab-IgM) ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: KTE62702

  • Size
  • Price
  • 48T $280
  • 96T $480
  • 96T*5 $2160
  • 96T*50 $20160

Contact information

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  •  Fax: +86-27-59716788
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