- Product name
HRP, Goat Anti-Mouse IgG
Mouse IgG whole molecule
ELISA, IHC, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10,000-1:100,000), IHC (1:500-1:2,000) and ELISA (1:5,000-1:100,000).
Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE.
- Storage buffer
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Most popular with customers
Application: FCM, ICC, IF
Application: IP, WB
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
TNF-α stimulates endothelial palmitic acid transcytosis and promotes insulin resistance
Li, Wenjing, et al. Scientific Reports 7 (2017).
Role of RAS/Wnt/β-catenin axis activation in the pathogenesis of podocyte injury and tubulo-interstitial nephropathy
Chen, Lin, et al. Chemico-Biological Interactions (2017).
Maintenance of the contractile phenotype in corpus cavernosum smooth muscle cells by Myocardin gene therapy ameliorates erectile dysfunction in bilateral cavernous nerve injury rats
Zhang, H.B., et al. Andrology (2017).
Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats
Wang, Jingfeng, et al. Evidence-Based Complementary and Alternative Medicine 2017 (2017).
CDKL1 promotes tumor proliferation and invasion in colorectal cancer
Qin, Chunzhi, et al. OncoTargets and therapy 10 (2017): 1613.
LAMC1 mRNA promotes malignancy of hepatocellular carcinoma cells by competing for MicroRNA-124 binding with CD151
Yang, Zhan‐Po, et al. IUBMB life (2017).
MicroRNA-384-mediated Herpud1 upregulation promotes angiotensin II-induced endothelial cell apoptosis
Lin, Hui, et al. Biochemical and Biophysical Research Communications 488.3 (2017): 453-460.
Effects of Total Flavone from Rhododendron simsii Planch. Flower on Postischemic Cardiac Dysfunction and Cardiac Remodeling in Rats
Cheng, Xinqi, et al. Evidence-Based Complementary and Alternative Medicine 2017 (2017).
Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model
Liu, Xiaoyu, et al. International journal of chronic obstructive pulmonary disease 12 (2017): 1309.
Sodium Salicylate Modulates Inflammatory Responses Through AMP-Activated Protein Kinase Activation in LPS-stimulated THP-1 Cells
Bao, Weiwei, et al. Journal of Cellular Biochemistry (2017).
Dual-functional nanoparticles for precise drug delivery to Alzheimer’s disease lesions: Targeting mechanisms, pharmacodynamics and safety
Zheng, Xiaoyao, et al. International journal of pharmaceutics 525.1 (2017): 237-248.
The Role of Spinal GABAB Receptors in Cancer-Induced Bone Pain in Rats
Zhou, Ya-Qun, et al. The Journal of Pain (2017).
Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen β-Conglycinin
Wang, Yao, et al. Food Analytical Methods (2017): 1-7.
Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway
Zheng, T, et al. Journal of Ethnopharmacology 193(2016): 691-699
A Novel Mechanism of Action for Salidroside to Alleviate Diabetic Albuminuria: Effects on Albumin Transcytosis across Glomerular Endothelial Cells
Wu, D, et al. American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237.
Suppression of endothelial PGC-1α is associated with hypoxia-induced endothelial dysfunction and provides a new therapeutic target in pulmonary arterial hypertension
Ye, Jia-Xin, et al. American Journal of Physiology-Lung Cellular and Molecular Physiology 310.11 (2016): L1233-L1242.
Preparation and Identification of the Artificial Antigen of Ivermectin
Wang Aiping, et al. Chinese Agricultural Science Bulletin, 31.17 (2015): 22-27.
CD137 regulates NFATc1 expression in mouse VSMCs through TRAF6/NF-κB p65 signaling pathway
Yan, Jinchuan, et al. Mediators of inflammation 2015 (2015).
The Endoribonuclease Activity Essential for the Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus to Inhibit NLRP3 Inflammasome-Mediated IL-1β Induction
Wang, Chao, et al. DNA and cell biology 34.12 (2015): 728-735.
Effect and mechanism of acidification on vascular calcification in chronic renal failure rats
Zhang Shenglei, et al. Chinese Journal of Nephrology,31.12 (2015): 924-931.
Bmi-1 promotes the invasion and migration of colon cancer stem cells through the downregulation of E-cadherin.
Zhang Z, Bu X, Chen H, et al. International Journal of Molecular Medicine, 2016, 38: 1199-1207.
Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells.
Du R, Liu Z, Hou X, et al. Molecular medicine reports, 2016, 13(6): 5045-5052.
High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli.
Jiang D, Liu Y, Wang A, et al. Biotechnology letters, 2016, 38(6): 901-908.
Electroacupuncture relieves labour pain and influences the spinal dynorphin/κ-opioid receptor system in rats.
Jiang Q Y, Wang M Y, Li L, et al. Acupuncture in Medicine, 2016, 34(3): 223-228.
IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB.
Gao Q, Liu Y, Wu Y, et al. International journal of molecular medicine, 2016, 37(2): 347-358.
Identification and BAC construction of Han, the first characterized HCMV clinical strain in China.
Zhao F, Shen Z Z, Liu Z Y, et al. Journal of medical virology, 2016, 88(5): 859-870.
Restoration of murine femoral segmental defect using CTGF-overexpressing MC3T3-E1 cells.
Huang X, Li Y, Xu J, et al. American journal of translational research, 2016, 8(3): 1530.
Construction and evaluation of the novel DNA vaccine harboring the inhibin α (1–32) and the RF-amide related peptide-3 genes for improving fertility in mice.
Dan X, Han L, Riaz H, et al. Experimental Animals, 2016, 65(1): 17.
The regulation of glycogen synthase kinase-3β on the mesenchymal stem cell therapy in the lypopolysaccharide induced acute lung injury in rat.
Lkhamsuren B, Wang H, Gao P, et al. Int J Clin Exp Med, 2016, 9(4): 7102-7114.
Decreased spermatogenesis led to alterations of testis-specific gene expression in male mice following nano-TiO2 exposure.
Hong F, Zhao X, Si W, et al. Journal of hazardous materials, 2015, 300: 718-728.
Salidroside improves endothelial function and alleviates atherosclerosis by activating a mitochondria-related AMPK/PI3K/Akt/eNOS pathway.
Xing S S, Yang X Y, Zheng T, et al. Vascular pharmacology, 2015, 72: 141-152.
Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes.
Shao L, Chian R C, Xu Y, et al. Reproduction, Fertility and Development, 2015.
Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3β pathway.
Zheng T, Yang X, Wu D, et al. British journal of pharmacology, 2015, 172(13): 3284-3301.
Beagle dogs have low susceptibility to BJ94-like H9N2 avian influenza virus.
Zhou P, Wang L, Huang S, et al. Infection, Genetics and Evolution, 2015, 31: 216-220.
Rapid and sensitive detection of the food allergen glycinin in powdered milk using a lateral flow colloidal gold immunoassay strip test.
Wang Y, Deng R, Zhang G, et al. Journal of agricultural and food chemistry, 2015, 63(8): 2172-2178.
TLR4 inhibitor resatorvid provides neuroprotection in experimental traumatic brain injury: implication in the treatment of human brain injury.
Zhang D, Li H, Li T, et al. Neurochemistry international, 2014, 75: 11-18.
In vitro and in vivo anti-cancer activities of Kuding tea (Ilex kudingcha C.J. Tseng) against oral cancer.
Zhu K, Li G, Sun P, et al. Experimental and therapeutic medicine, 2014, 7(3): 709-715.