EliKine™ Testosterone ELISA Kit

Product Information

  • Product name

    EliKine™ Testosterone ELISA Kit

  • Reactivity

    Human, Mouse

  • Applications

    ELISA

  • Application notes

    EliKine™ Testosterone ELISA Kit employs the competitive inhibition enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for testosterone and Horseradish Peroxidase (HRP) conjugated testosterone. The competitive inhibition reaction is launched between with HRP labeled testosterone and unlabeled testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of testosterone in the sample. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Other biological fluids, Plasma, Serum

  • Assay type

    Competitive ELISA (quantitative)

  • Assay duration

    Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.

Fig.1. Testosterone Standard Curve.

Fig.2. EliKine™ Testosterone ELISA Kit is a competitive ELISA to quantitate Testosterone in samples.

Product Properties

  • Kit components

    • Testosterone microplate
    • Testosterone standard
    • HRP conjugated testosterone
    • Testosterone detect antibody
    • HRP substrate A
    • HRP substrate B
    • Stop solution
    • Wash buffer
    • Plate covers.

  • Features & Benefits

    EliKine™ Testosterone ELISA Kit has high sensitivity and excellent specificity for detection of Testosterone. No significant cross-reactivity or interference between Testosterone and analogues was observed.

  • Calibration range

    0.1 ng/ml-20 ng/ml

  • Limit of detection

    0.1 ng/ml

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for

  • Storage instructions

    The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Testosterone is the primary male sex hormone and an anabolic steroid. In men, testosterone plays a key role in the development of male reproductive tissues such as the testis and prostate, as well as promoting secondary sexual characteristics such as increased muscle and bone mass, and the growth of body hair. In addition, testosterone is involved in health and well-being, and the prevention of osteoporosis. Insufficient levels of testosterone in men may lead to abnormalities including frailty and bone loss. Testosterone is a steroid from the androstane class containing a keto and hydroxyl groups at the three and seventeen positions respectively. It is biosynthesized in several steps from cholesterol and is converted in the liver to inactive metabolites. It exerts its action through binding to and activation of the androgen receptor.

  • Alternative names

    17ß-hydroxyandrost-4-ene-3-one

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EliKine™ Testosterone ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KET0001

  • Size
  • Price
  • 48T $140
  • 96T $220
  • 96T*5 $1000

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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