EliKine™ Human Prolactin ELISA Kit

Product Information

  • Product name

    EliKine™ Human Prolactin ELISA Kit

  • Reactivity

    Human, Mouse

  • Applications

    ELISA

  • Application notes

    EliKine™ Human Prolactin ELISA Kit employs a two-site sandwich ELISA to quantitate Prolactin in samples. An antibody specific for prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any prolactin present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for prolactin is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.

Fig.1. Human Prolactin Standard Curve.

Fig.2. EliKine™ Human Prolactin ELISA Kit is a sandwich ELISA to quantitate Prolactin in samples.

Product Properties

  • Kit components

    • Human Prolactin microplate
    • Human Prolactin standard
    • Human Prolactin detect antibody
    • HRP substrate A
    • HRP substrate B
    • Stop solution
    • Wash buffer
    • Plate covers.

  • Features & Benefits

    EliKine™ Human Prolactin ELISA Kit has high sensitivity and excellent specificity for detection of Human Prolactin. No significant cross-reactivity or interference between Human Prolactin and analogues was observed.

  • Calibration range

    40 μIU/ml-2000 μIU/ml

  • Limit of detection

    50 μIU/ml

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for

  • Storage instructions

    The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin recepto. Although better characterized in rodents than humans, post-translational modifcations such as polymerization, glycosylation, and proteolytic cleavage can alter the activities of prolactin. Non-glycosylated prolactin is produced by the pituitary gland and packaged in storage granules before secretion. Glycosylated prolactin is reported to be constitutively secreted, have lower biological potency, and be removed from the circulation more quickly.

  • Gene ID

    5617

  • Alternative names

    PRL; Prl1a1; Prol; Prolactin precursor; RATPRLSD1; RNPROL; RP23-351I6.2; RP3-404K8.1; OTTMUSP00000000651; Luteotropic Hormone; Lactogenic Hormone; Mammotropin; AV290867

  • Accession

    P01236

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EliKine™ Human Prolactin ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KET6904

  • Size
  • Price
  • 48T $140
  • 96T $220
  • 96T*5 $1000

Contact information

Do not hesitate to contact us if you have any questions.

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  •  Fax: +86-27-59716788
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