EliKine™ Human LH ELISA Kit

Product Information

  • Product name

    EliKine™ Human LH ELISA Kit

  • Reactivity

    Human

  • Applications

    ELISA

  • Application notes

    EliKine™ Human LH ELISA Kit employs a two-site sandwich ELISA to quantitate LH in samples. An antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for LH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.

Fig.1. Human LH Standard Curve.

Fig.2. EliKine™ Human LH ELISA Kit is a sandwich ELISA to quantitate LH in samples.

Product Properties

  • Kit components

    • Human LH microplate
    • Human LH standard
    • Human LH detect antibody
    • HRP substrate A
    • HRP substrate B
    • Stop solution
    • Wash buffer
    • Plate covers.

  • Features & Benefits

    EliKine™ Human LH ELISA Kit has high sensitivity and excellent specificity for detection of Human LH. No significant cross-reactivity or interference between Human LH and analogues was observed.

  • Calibration range

    2 IU/L-75 IU/L

  • Limit of detection

    0.5 IU/L

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormonereleasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production.

  • Gene ID

    3972

  • Alternative names

    LH-B; LHB; LSH-B; LSH-beta; LSHB_HUMAN; Luteinizing hormone subunit beta; Lutropin beta chain; Lutropin subunit beta

  • Accession

    P01229

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EliKine™ Human LH ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KET6902

  • Size
  • Price
  • 48T $140
  • 96T $220
  • 96T*5 $1000

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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