EliKine™ Free Triiodothyronine (fT3) ELISA Kit

Product Information

  • Product name

    EliKine™ Free Triiodothyronine (fT3) ELISA Kit

  • Reactivity

    Human, Mouse

  • Applications

    ELISA

  • Application notes

    EliKine™ fT3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT3. A competitive inhibition reaction is launched between fT3 (Standards or samples) and Biotin-conjugated fT3 with the pre-coated antibody specific for fT3. The more amount of fT3 in samples, the less antibody bound by Biotin-conjugated fT3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT3 in the sample. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Other biological fluids, Plasma, Serum

  • Assay type

    Competitive ELISA (quantitative)

  • Assay duration

    Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.

Fig.1. fT3 Standard Curve.

Fig.2. EliKine™ fT3 ELISA Kit is a competitive ELISA to quantitate fT3 in samples.

Product Properties

  • Kit components

    • fT3 microplate
    • fT3 standard
    • Biotin conjugated fT3
    • Avidin-HRP
    • HRP substrate A
    • HRP substrate B
    • Stop solution
    • Wash buffer
    • Plate covers.

  • Features & Benefits

    EliKine™ fT3 ELISA Kit has high sensitivity and excellent specificity for detection of fT3. No significant cross-reactivity or interference between fT3 and analogues was observed.

  • Calibration range

    2 pmol/L-32 pmol/L

  • Limit of detection

    2.0 pmol/L

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for

  • Storage instructions

    The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Over 99% of Triiodothyronine (T3) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T3 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T3 level changes so that the free T3 concentration remains constant. Thus, measurements of free T3 concentrations correlate more reliably with clinical status than total T3 levels. The increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains basically unchanged.

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EliKine™ Free Triiodothyronine (fT3) ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KET0004

  • Size
  • Price
  • 48T $140
  • 96T $220
  • 96T*5 $1000

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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