Cleaved-PARP-1 (D214) Polyclonal Antibody

Product Information

  • Product name

    Cleaved-PARP-1 (D214) Polyclonal Antibody

  • Immunogen

    Synthesized peptide derived from the Internal region of human PARP-1. at AA rangle: 140-220

  • Host

    Rabbit

  • Reactivity

    Human, Mouse

  • Applications

    ELISA, IF, IHC-p, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:20000. Not yet tested in other applications.

  • Clonality

    Polyclonal

  • Isotype

    Rabbit IgG

  • Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Fig.1 Western Blot analysis of MCF-7 (1), 293T (2), HELA (3), diluted at 1:2000.

Fig.2 Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3 Immunohistochemical analysis of paraffin-embedded mouse kidney tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4 Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5 Immunofluorescence analysis of human stomach cancer tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

Fig.6 Immunofluorescence analysis of mouse spleen tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

Fig.7 Immunofluorescence analysis of rat spleen tissue. 1, Cleaved-PARP-1 (D214) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Product Properties

  • Formulation

    Liquid solution

  • Concentration

    1 mg/ml

  • Storage buffer

    PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    PARP1 encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, poly(ADP-ribose) polymerase 1 may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

  • Gene ID

    142

  • Alternative names

    PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

  • Others

    Cleaved-PARP-1 (D214) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to D214.

  • Accession

    P09874

Most popular with customers

Reviews

There are no reviews yet.

Be the first to review “Cleaved-PARP-1 (D214) Polyclonal Antibody”

Your email address will not be published. Required fields are marked *

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: ABP50072

  • Size
  • Price
  • 30μl $100
  • 100μl $220
  • 200μl $360

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

Gaining Your Trust with our

High quality *
Attractive price
100% Guarantee
Secured shipment