Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

Product Information

  • Product name

    Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

  • Immunogen

    Recombinant Protein

  • Host

    Mouse

  • Reactivity

    Plants

  • Applications

    WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:2000-5000.

  • Clonality

    Monoclonal

  • Isotype

    Mouse IgG1

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Fig.Western blot analysis (1:3,000) of plant Actin expression in Arabidopsis (lane A) and Nicotiana tabacum (lane B) with Anti-Plant Actin mouse monoclonal antibody (3T3).

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.

  • Gene ID

    820385

  • Alternative names

    AT3G12110; actin-11

  • Accession

    P53496

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

  1. The Arabidopsis F-box E3 ligase RIFP1 plays a negative role in abscisic acid signalling by facilitating ABA receptor RCAR3 degradation.

    Li Y, Zhang L, Li D, et al. Plant, cell & environment, 2016, 39(3): 571-582.

  2. The Protein Elicitor PevD1 Enhances Resistance to Pathogens and Promotes Growth in Arabidopsis.

    Liu M, Khan N U, Wang N, et al. International Journal of Biological Sciences, 2016, 12(8): 931.

  3. Fungal cellulase is an elicitor but its enzymatic activity is not required for its elicitor activity

    Ma Y, Han C, Chen J, et al. Molecular plant pathology, 2015, 16(1): 14-26.

Cat #: A01050

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860
  • 10ml $4000

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