Caspase-9, which is activated by association with the Apaf-1 (apoptotic protease-activating factor-1) apoptosome complex, cleaves and activates the downstream effector caspases-3 and -7, thereby executing the caspase-cascade and cell-death programme. Although caspase-9 does not need to be cleaved to be active, apoptotic cell death is always accompanied by autocatalytic cleavage and by further downstream effector caspase-dependent cleavage of caspase-9. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Abbkine Cleaved-Caspase-9 (D353) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the C-terminal region of human Caspase-9 at AA rangle: 280-360. The antibody is react with mouse and rat and the verified applications are WB, IHC-P and ELISA. Abbkine suggested the starting dilutions are WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:20000.
Supplied as liquid , the concentration of the Cleaved-Caspase-9 (D353) antibody is 1mg/ml. Uniprot accession of the relative protein of the antibody is P55211, and gene ID is 842. The antibody is stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.