Easy to deal with Immunoprecipitation, "IP new weapon" is coming
1.Background principle: Immunoprecipitation refers to a method of preliminarily separating the target protein by precipitating the antigen (usually the target protein) from the mixed system by using the characteristic that the antibody can specifically bind to the antigen. Immunoprecipitation is developed based on the traditional affinity purification method. 2.Questions: Although the principle is not difficult, the result is not easy. 1. How many researchers are struggling in the sea of searching for the target strips, 2. How many people are foolishly unable to distinguish the strips under the interference of light and heavy chains; 3. How many people are struggling "Lost yourself" in complex manipulation experiments. Solution: Abbkine has launched a series of IP toolboxes to directly address the…
Encyclopedia of biochemical detection indicators
1、 Definition of biochemical detection Biochemical testing refers to the use of biological or chemical methods to test samples. The determination indexes are usually biochemical indexes such as enzymes, carbohydrates, lipids, proteins and non-protein nitrogen, inorganic elements or body function indexes. In scientific research, biochemical detection provides various conveniences for material content determination, enzyme activity rapid detection and drug development. 2、 Classification of biochemical detection indexes a.Oxidation and Antioxidation Series Oxidation is a common chemical reaction in life, free radicals are produced in the process of oxidation, and antioxidation is a molecule that scavenges free radicals in the body. Enzymatic and non-enzymatic antioxidants play a crucial role in protecting against peroxidative damage caused by exercise. Common oxidation and antioxidant series detection indicators mainly include…
Protein Quantification Topic|What protein quantification methods have you used over these years?
Protein quantification is an important step that must be taken before protein isolation and analysis, protein chromatography separation, protein electrophoresis analysis, protein immunosegregation and analysis, total protein quantification of cell split interpretation, labeling of protein molecules, protein structure analysis, etc., all need reliable quantitative analysis as the basis. The fields of application of protein quantification analysis include biological sciences, food testing, clinical testing, and more. Specifically, we need to use protein quantification experiments in the following scenarios: 1. When judging whether the protein is expressed, first lyse the cells to verify whether the cell lysis is thorough, or to carry out the usual experiment or standardized preservation of the sample, then the cell lysate should be quantified for protein; 2.…
Universal IF Toolkit|Arrange the immunofluorescence experiment clearly
When it comes to immunohistochemistry, as the name suggests, immunohistochemistry (IHC) uses antibodies to detect the localization of proteins and other antigens in tissue slices. Antibody-antigen interactions are detected by enzyme substrate colorimetry or by fluorescent dyes. Although IHC is not as quantitative as Western blotting or ELISA, it can provide valuable information on protein localization in the complete tissue background. Protein expression profiling is of great value to pathologists and can also be used as an important diagnostic tool. The key to the success of IHC experiments lies in a stable, optimized, and reproducible staining protocol that uses high-quality specific reagents. Here we will briefly talk about a major branch of immunohistochemistry immunofluorescence (IF), immunofluorescence experiments seem simple, but…
Beyond BrdU, a new generation of cell proliferation imaging analysis kit (EdU method)
1、 What is EDU? EdU, named as 5-ethynyl-2-deoxyuridine, is a nucleoside analogue of thymine. Its alkyne groups are rare in natural compounds and can replace thymine (T) to infiltrate into the synthetic DNA molecule during the period of DNA replication for DNA synthesis. It is widely used in experimental researches such as cell proliferation. 2、 What is BrdU? The name of BrdU is 5-bromo-2 ' -deoxyuridine. BrdU is a synthetic thymine analogue of thymidine, which is incorporated into the newly synthesized genomic DNA in the mitotic S phase. Subsequently, DNA denaturation was performed to determine BrdU insertion and any other cellular surface and / or intracellular target of interest through cell staining. 3、 Detection principle of Edu EdU can be incorporated into the newly…
Immunofluorescence Master Key - Universal IF Toolkit
We usually encounter many difficult problems when doing immunofluorescence experiments, such as weak staining or no signal, high background and strong non-specific signal, and for example, the hard work of slices, which was discovered only during the final observation. And for example, all the reagents for immunofluorescence are self-prepared, and the configuration time is also real. So how to solve these very common problems? Let's take a look. Immunofluorescence technology (IF) is based on the principle of antigen-antibody reaction. First, a known antigen or antibody is labeled with fluorescein to make a fluorescent antibody, and then the fluorescent antibody (or antigen) is used as a probe to detect tissue or cells. the corresponding antigen (or antibody). Based on the tedious…
Distinction between apoptosis, Pyroptosis, necrosis, iron death and autophagy
“To be, or not to be, that is the question”， To live or die, cells have to choose not only whether to die, but also how to die. There are many "patterns" of cell death, such as apoptosis, Pyroptosis, Ferroptosis and so on. Today, let's talk about cell death. What is apoptosis? Apoptosis is a fundamental biological phenomenon of cells that plays an essential role in the removal of unwanted or abnormal cells in multicellular organisms. It plays an important role in the evolution of organisms, the stability of the internal environment and the development of multiple systems. Apoptosis is not only a special type of cell death, but also has important biological significance and complex molecular biological mechanisms. Apoptosis is a…
WB|Universal WB Toolkit arranges the WB experiment well
Western Blotting is a powerful technique that allows you to easily detect proteins and estimate their expression and molecular weight. Samples start with a mixture of proteins extracted from cells or tissues. The complete protein western blot assay process consists of several separate steps, each of which is critical to the final data quality. In the actual operation process, the following problems are often encountered: 1.The sample pyrolysis effect is poor, the sample contains more DNA and cell particle impurities interference; 2.Reagent variety, and difficult configuration, after repeated use; 3.Lack of WB positive control, abnormal results can not determine the operation or sample problems; 4.ECL luminescence solution is not sensitive enough to show clear bands of low abundance proteins. Here…
Three minutes to take you through the problem of immunofluorescence
Immunofluorescence (IF) is a common experiment in immunology experiments. For the experimental masters, it may be the effort of sprinkling water, and a "dazzling and beautiful" film will come out, but for the novice operator, it may be in the overall process. It is not very friendly, and there will still be situations such as fluorescent bright spots in the entire cell sheet, or weak or no signal, too few stained cells, or only the blue light of DAPI in the observation field, and the target fluorescence has almost no or no signal. If so, it might be very frustrating. Today, let's walk into the world of immunofluorescence and see how to sort out our experiments. 1.Principle Immunofluorescence is a…
New products have been added to fatty acid metabolism series
Previously, we provided detailed knowledge about fatty acid metabolism, including fatty acid anabolism and catabolism, and also introduced two biochemical indicators related to fatty acid metabolism, including： 1.fatty acid synthase Fatty acid synthase (FAS) is a multifunctional homodimeric enzyme protein that is the primary enzyme required for the anabolic conversion of dietary carbohydrates to fatty acids. Fatty acid synthase catalyzes the conversion of acetyl coenzyme A and malonyl coenzyme A to long-chain saturated fatty acids in the presence of NADPH. Human fatty acid synthase is a large homodimeric multifunctional enzyme for the synthesis of palmitic acid. The unique carboxy-terminal thioesterase structural domain of fatty acid synthase hydrolyzes the growing fatty acid chains and plays a key role in regulating the…
